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Image Search Results
Journal: Aging (Albany NY)
Article Title: The neuroprotective effects of SIRT1 in mice carrying the APP/PS1 double-transgenic mutation and in SH-SY5Y cells over-expressing human APP670/671 may involve elevated levels of α7 nicotinic acetylcholine receptors
doi: 10.18632/aging.102713
Figure Lengend Snippet: Effects of RSV and suramin on the expression of synapse makers, p-CREB, GFAP as well as Iba-1, in mice carrying the APP/PS1 double mutation. ( A ) the level of SYP protein; ( B ) SNAP-25; ( C ) p-CREB; ( D ) GFAP and ( E ) Iba-1. The values presented are means ± SD. * P <0.05 compared with the WT group; # P <0.05 compared with the APP/PS1 group, as determined by analysis of variance (ANOVA), followed by the Tukey HSD test. Representative western blots are shown beneath D and F . Representative western blots are shown beneath each bar graph.
Article Snippet: RSV, Suramin, U0126 and the SIRT1 Assay Kit (Sigma-Aldrich, USA); methyllycaconitine (MLA) (Tocris Bioscience, UK); TRIzol reagent (Invitrogen, China), SYBR Green PCR master mix (Applied Biosystems, USA); ECL Plus reagent (Merck Millipore, Germany); the near-infrared amyloid-β fluorescent CRANAD-58 probe (ab146926, Abcam Inc., USA); rabbit monoclonal anti-p-Erk1/2 and rabbit monoclonal anti-Erk1/2 antibodies (4370 and 4695, Cell Signaling Technology (CST) Inc., USA); rabbit monoclonal anti-SIRT1 antibody and anti-SYP (synaptophysin) (ab12193 and ab8049; Abcam Inc., USA); rabbit polyclonal anti-α7 nAChR and mouse monoclonal anti-β-actin antibodies (sc-58607 and sc-376421, Santa Cruz Inc., USA); rabbit polyclonal anti SNAP-25 (synaptosomal-associated protein 25), rabbit polyclonal anti
Techniques: Expressing, Mutagenesis, Western Blot